HA-Tag antibody

Catalog No. ABIN3029804

Product Details anti-HA-Tag Antibody

Target: HA-Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Sequence: YPYDVPDYA

Purification: Purified

Immunogen: KLH conjugated synthetic peptide cYPYDVPDYA was used as the immunogen for the anti-HA antibody.

Clone: 12CA5

Isotype: IgG2b

Application Details

Application Notes: Titration of the anti-HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000, Immunoprecipitation: published application

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the anti-HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA tag (HA-Tag Products)

Target Type: Tag

Background: Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. NSJ's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.