Animal-Free
Reactivity: Tag
WB, ELISA, IF
Host: Mouse
Monoclonal
AB158-1-D11
unconjugated
Recombinant Antibody
Application Notes
Titration of the anti-HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000, Immunoprecipitation: published application
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the anti-HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
HA-Tag
Alternative Name
HA tag
Target Type
Tag
Background
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. NSJ's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.