ATG7 antibody (AA 284-313)

Catalog No. ABIN3029987

The Rabbit Polyclonal anti-ATG7 antibody has been validated for WB, IHC and ELISA. It is suitable to detect ATG7 in samples from Human and Mouse.

Quick Overview for ATG7 antibody (AA 284-313) (ABIN3029987)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ATG7 Antibody

Binding Specificity: AA 284-313

Cross-Reactivity (Details): Expected species reactivity: Rat, Chicken

Purification: Purified

Immunogen: A portion of amino acids 284-313 from the human protein was used as the immunogen for this APG7 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the APG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the APG7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (APG7)

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy