Axin antibody (AA 330-359)

Catalog No. ABIN3030088

This Rabbit Polyclonal antibody specifically detects Axin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Axin antibody (AA 330-359) (ABIN3030088)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Axin Antibody

Binding Specificity: AA 330-359

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 330-359 from the human protein was used as the immunogen for this AXIN1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the AXIN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the AXIN1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: AXIN1

Background: AXIN1 is a component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects, down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. [UniProt]

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling