beta Actin antibody

Catalog No. ABIN3029837

The Mouse Monoclonal anti-beta Actin antibody is suitable to detect beta Actin in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for beta Actin antibody (ABIN3029837)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 137CT26-1-1

Product Details anti-beta Actin Antibody

Cross-Reactivity (Details): Expected species reactivity: Rat, Bovine, Pig, Primate, Chicken, Xenopus

Purification: Purified

Immunogen: This b-Actin antibody was produced from a mouse immunized with ACTB recombinant protein.

Isotype: IgG1 kappa

Application Details

Application Notes: Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the b-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: B-Actin

Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction