Caspase 3 antibody (AA 149-179)
Quick Overview for Caspase 3 antibody (AA 149-179) (ABIN3030296)
Target
See all Caspase 3 (CASP3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 149-179
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Purification
- Antigen affinity purified
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Immunogen
- A portion of amino acids 149-179 from the human protein was used as the immunogen for this Caspase-3 antibody.
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Isotype
- Ig Fraction
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Application Notes
- Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- In 1X PBS pH 7.4 with 0.09 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Aliquot the Caspase-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Caspase 3 (CASP3)
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Alternative Name
- Caspase-3
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Background
- This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
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UniProt
- P42574
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Target
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