CD28 antibody (AA 182-208)

Catalog No. ABIN3028547

The Rabbit Polyclonal anti-CD28 antibody has been validated for WB, IF and ELISA. It is suitable to detect CD28 in samples from Human.

Quick Overview for CD28 antibody (AA 182-208) (ABIN3028547)

Target: CD28

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-CD28 Antibody

Binding Specificity: AA 182-208

Cross-Reactivity (Details): Expected species reactivity: Rabbit

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 182-208 from the human protein was used as the immunogen for this CD28 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CD28 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CD28 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response