Titration of the CDC20 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the CDC20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
CDC20
(Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.