EED antibody (AA 269-303)

Catalog No. ABIN3030801

This anti-EED antibody is a Rabbit Polyclonal antibody detecting EED in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for EED antibody (AA 269-303) (ABIN3030801)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EED Antibody

Binding Specificity: AA 269-303

Purification: Antigen affinity purified

Immunogen: This EED antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 269-303 amino acids from the central region of mouse Eed.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the EED antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the EED antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED

Background: Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.

UniProt: Q921E6