Histone H3.3 antibody (Lys9)

Catalog No. ABIN3031135

This Rabbit Polyclonal antibody specifically detects Histone H3.3 in ELISA, WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Histone H3.3 antibody (Lys9) (ABIN3031135)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Histone H3.3 Antibody

Binding Specificity: Lys9

Purification: Antigen affinity purified

Immunogen: This Histone H3.3 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human H3F3A.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Histone H3.3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Histone H3.3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3 (H3F3A)

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

UniProt: P84243