MYB antibody (AA 506-534)

Catalog No. ABIN3029530

This Rabbit Polyclonal antibody specifically detects MYB in WB, ELISA and IHC. It exhibits reactivity toward Mouse.

Quick Overview for MYB antibody (AA 506-534) (ABIN3029530)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MYB Antibody

Binding Specificity: AA 506-534

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 506-534 from the human protein was used as the immunogen for this Myb antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Myb antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:50-1:100,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Myb antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: Myb

Background: Myb is a transcriptional activator, DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.

UniProt: P10242

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process