Nanog antibody (AA 15-49)

Catalog No. ABIN3031942

This Rabbit Polyclonal antibody specifically detects Nanog in WB, ELISA, IF, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Nanog antibody (AA 15-49) (ABIN3031942)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Nanog Antibody

Binding Specificity: AA 15-49

Cross-Reactivity (Details): Expected species reactivity: Primate

Purification: Purified

Immunogen: A portion of amino acids 15-49 from the human protein was used as the immunogen for this NANOG antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. [UniProt]

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance