Nestin antibody (AA 1389-1416)

Catalog No. ABIN3031961

The Rabbit Polyclonal anti-Nestin antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect Nestin in samples from Human.

Quick Overview for Nestin antibody (AA 1389-1416) (ABIN3031961)

Target: Nestin (NES)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Nestin Antibody

Binding Specificity: AA 1389-1416

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Nestin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

UniProt: P48681