Nucleolin antibody

Catalog No. ABIN3032072

The Mouse Monoclonal anti-Nucleolin antibody has been validated for WB, IHC, IF and ELISA. It is suitable to detect Nucleolin in samples from Human.

Quick Overview for Nucleolin antibody (ABIN3032072)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: 130CT43-8-5

Product Details anti-Nucleolin Antibody

Purification: Purified

Immunogen: This Nucleolin antibody was produced from mice immunized with a protein from human NCL.

Isotype: IgG1

Application Details

Application Notes: Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Nucleolin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization