c-FOS antibody (pThr232)

Catalog No. ABIN3030222

This anti-c-FOS antibody is a Rabbit Polyclonal antibody detecting c-FOS in ELISA and DB. Suitable for Human.

Quick Overview for c-FOS antibody (pThr232) (ABIN3030222)

Target: c-FOS (c-Fos) (c-Fos)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-c-FOS Antibody

Binding Specificity: pThr232

Purification: Antigen affinity purified

Immunogen: This phospho-c-Fos antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT232 of the human protein.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-c-Fos antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-c-Fos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: C-Fos

Background: C-Fos is a nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. [UniProt]

UniProt: P01100

Pathways: S100 Proteins