Caspase 3 antibody (pSer26)

Catalog No. ABIN3030304

This Rabbit Polyclonal antibody specifically detects Caspase 3 in ELISA and DB. It exhibits reactivity toward Mouse.

Quick Overview for Caspase 3 antibody (pSer26) (ABIN3030304)

Target: Caspase 3 (CASP3)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-Caspase 3 Antibody

Binding Specificity: pSer26

Purification: Antigen affinity purified

Immunogen: This phospho-Caspase-3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS26 of mouse CASP3.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-Caspase-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].

UniProt: P70677

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation