ENOS antibody (pSer1177)

Catalog No. ABIN3032005

This Rabbit Polyclonal antibody specifically detects ENOS in ELISA, IF and DB. It exhibits reactivity toward Human.

Quick Overview for ENOS antibody (pSer1177) (ABIN3032005)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-ENOS Antibody

Binding Specificity: pSer1177

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Pig

Purification: Antigen affinity purified

Immunogen: This phospho-eNos antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1177 of human eNos.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-eNos antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-eNos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: eNOS

Background: ENos produces nitric oxide, a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling