OCT4 antibody (pSer236)

Catalog No. ABIN3032291

The Rabbit Polyclonal anti-OCT4 antibody has been validated for ELISA and DB. It is suitable to detect OCT4 in samples from Human.

Quick Overview for OCT4 antibody (pSer236) (ABIN3032291)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-OCT4 Antibody

Binding Specificity: pSer236

Cross-Reactivity (Details): Expected species reactivity: Mouse, Bovine, Pig

Purification: Antigen affinity purified

Immunogen: This phospho-Oct4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS236 of human OCT4.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-Oct4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct4

Background: OCT4 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

UniProt: Q01860

Pathways: Stem Cell Maintenance