OCT4 antibody (AA 214-247)

Catalog No. ABIN3028942

This Rabbit Polyclonal antibody specifically detects OCT4 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for OCT4 antibody (AA 214-247) (ABIN3028942)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-OCT4 Antibody

Binding Specificity: AA 214-247

Purification: Antigen affinity purified

Immunogen: This mouse Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 214-247 amino acids from the Central region of mouse Pou5f1.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:4000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Pou5f1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Pou5f1

Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

UniProt: P20263

Pathways: Stem Cell Maintenance