PSMA antibody (AA 161-190)

Catalog No. ABIN3030978

This anti-PSMA antibody is a Rabbit Polyclonal antibody detecting PSMA in WB, IHC, ELISA and IF. Suitable for Human.

Quick Overview for PSMA antibody (AA 161-190) (ABIN3030978)

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PSMA Antibody

Binding Specificity: AA 161-190

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Pig

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 161-190 from the human protein was used as the immunogen for this PSMA antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PSMA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PSMA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Alternative Name: PSMA

Background: GCPII is a zinc metalloenzyme that resides in membranes. Most of the enzyme resides in the extracellular space. GCPII is a class II membrane glycoprotein. It catalyzes the hydrolysis of N-acetylaspartylglutamate (NAAG) to glutamate and N-acetylaspartate (NAA). Neuroscientists primarily use the term NAALADase in their studies, while those studying folate metabolism use folate hydrolase, and those studying prostate cancer or oncology, PSMA. All of which refer to the same protein, glutamate carboxypeptidase II. PSMA is strongly expressed in the human prostate, being a hundredfold greater than the expression in most other tissues. In cancer, it is upregulated in expression and has been called the second-most-upregulated gene in prostate cancer, with increase of 8- to 12-fold over the noncancerous prostate. In human prostate cancer, the higher expressing tumors are associated with quicker time to progression and a greater percentage of patients suffering relapse. [Wiki]

UniProt: Q04609