DDX58 antibody (AA 894-925)

Catalog No. ABIN3030701

The Rabbit Polyclonal anti-DDX58 antibody has been validated for WB and ELISA. It is suitable to detect DDX58 in samples from Human and Mouse.

Quick Overview for DDX58 antibody (AA 894-925) (ABIN3030701)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX58 Antibody

Binding Specificity: AA 894-925

Purification: Purified

Immunogen: A portion of amino acids 894-925 from the human protein was used as the immunogen for this RIG-I antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the RIG-I antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RIG-I antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: RIG-I

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, RIG-I triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). This protein is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C