SMAD3 antibody (AA 186-215)

Catalog No. ABIN3032621

The Rabbit Polyclonal anti-SMAD3 antibody has been validated for WB, ELISA, IHC, IF and FACS. It is suitable to detect SMAD3 in samples from Human and Mouse.

Quick Overview for SMAD3 antibody (AA 186-215) (ABIN3032621)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 186-215

Cross-Reactivity (Details): Expected species reactivity: Rat, Pig, Chicken

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 186-215 from the human protein was used as the immunogen for this SMAD3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SMAD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy