SP1 antibody (AA 677-707)

Catalog No. ABIN3032701

This Rabbit Polyclonal antibody specifically detects SP1 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for SP1 antibody (AA 677-707) (ABIN3032701)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SP1 Antibody

Binding Specificity: AA 677-707

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 677-707 from the human protein was used as the immunogen for this SP1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. [UniProt]

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction