SPARC antibody (AA 224-251)

Catalog No. ABIN3032525

The Rabbit Polyclonal anti-SPARC antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect SPARC in samples from Human.

Quick Overview for SPARC antibody (AA 224-251) (ABIN3032525)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-SPARC Antibody

Binding Specificity: AA 224-251

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Xenopus

Purification: Purified

Immunogen: A portion of amino acids 224-251 from the human protein was used as the immunogen for this SPARC antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SPARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPARC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background: Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).

UniProt: P09486

Pathways: Autophagy