SREBF2 antibody (AA 399-427)

Catalog No. ABIN3032752

This Rabbit Polyclonal antibody specifically detects SREBF2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for SREBF2 antibody (AA 399-427) (ABIN3032752)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SREBF2 Antibody

Binding Specificity: AA 399-427

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 399-427 from the human protein was used as the immunogen for this SREBP2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SREBP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SREBP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBP2

Background: SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.

UniProt: Q12772

Pathways: Regulation of Lipid Metabolism by PPARalpha