The Rabbit Polyclonal anti-SUMO antibody has been validated for ELISA and WB. It is suitable to detect SUMO in samples from Drosophila melanogaster and Human.
Titration of the SUMO antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the SUMO antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
SUMO
Background
Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. In the development of Drosophila, SUMO plays a maternal role in anterior-posterior (A/P) polarity and patterning.