TLR5 antibody (AA 825-856)

Catalog No. ABIN3029173

This Rabbit Polyclonal antibody specifically detects TLR5 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for TLR5 antibody (AA 825-856) (ABIN3029173)

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-TLR5 Antibody

Binding Specificity: AA 825-856

Purification: Purified

Immunogen: A portion of amino acids 825-856 from the mouse protein was used as the immunogen for this TLR5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the TLR5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the TLR5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for TLR5

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Alternative Name: TLR5

Background: TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. It also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.

UniProt: Q9JLF7

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades