UBE2I antibody (AA 1-30)

Catalog No. ABIN3029438

The Rabbit Polyclonal anti-UBE2I antibody has been validated for WB, IHC and ELISA. It is suitable to detect UBE2I in samples from Human.

Quick Overview for UBE2I antibody (AA 1-30) (ABIN3029438)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-UBE2I Antibody

Binding Specificity: AA 1-30

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Chicken, Zebrafish, Xenopus

Purification: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this UBC9 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the UBC9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the UBC9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBC9

Background: UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway