Ubiquitin antibody (N-Term)

Catalog No. ABIN3029416

This Rabbit Polyclonal antibody specifically detects Ubiquitin in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Ubiquitin antibody (N-Term) (ABIN3029416)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ubiquitin Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): Expected species reactivity: Bovine, C. elegans, Chicken, Drosophila, Hamster, Pig, Primate, Rabbit, Xenopus

Purification: Antigen affinity purified

Immunogen: This Ubiquitin antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57amino acids from the N-terminal region of the human protein.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Ubiquitin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway