Ubiquitin antibody (AA 36-66)

Catalog No. ABIN3029420

The Rabbit Polyclonal anti-Ubiquitin antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect Ubiquitin in samples from Human.

Quick Overview for Ubiquitin antibody (AA 36-66) (ABIN3029420)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Ubiquitin Antibody

Binding Specificity: AA 36-66

Cross-Reactivity (Details): Expected species reactivity: Bovine, C. elegans, Chicken, Drosophila, Mouse, Pig, Primate, Rat,Yeast

Purification: Purified

Immunogen: A portion of amino acids 36-66 from the human protein was used as the immunogen for this Ubiquitin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Ubiquitin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: This gene encodes Ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

UniProt: P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway