UCHL5 antibody (AA 56-87)

Catalog No. ABIN3029428

This Rabbit Polyclonal antibody specifically detects UCHL5 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for UCHL5 antibody (AA 56-87) (ABIN3029428)

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-UCHL5 Antibody

Binding Specificity: AA 56-87

Cross-Reactivity (Details): Expected species reactivity: Bovine, Pig

Purification: Purified

Immunogen: A portion of amino acids 56-87 from the human protein was used as the immunogen for this UCH37 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the UCH37 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the UCH37 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for UCHL5

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Alternative Name: UCH37

Background: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.

UniProt: Q9Y5K5