WT1 antibody (AA 346-375)

Catalog No. ABIN3029622

This anti-WT1 antibody is a Rabbit Polyclonal antibody detecting WT1 in IHC, IF, FACS, WB and ELISA. Suitable for Human.

Quick Overview for WT1 antibody (AA 346-375) (ABIN3029622)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), ELISA

Product Details anti-WT1 Antibody

Binding Specificity: AA 346-375

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Pig, Xenopus

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 346-375 from the human protein was used as the immunogen for this WT1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the WT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the WT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 (Wilms Tumor Protein)

Background: Wilms Tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

UniProt: P19544

Pathways: Tube Formation