XBP1 antibody (AA 62-91)

Catalog No. ABIN3029642

The Rabbit Polyclonal anti-XBP1 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect XBP1 in samples from Human.

Quick Overview for XBP1 antibody (AA 62-91) (ABIN3029642)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-XBP1 Antibody

Binding Specificity: AA 62-91

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 62-91 from the human protein was used as the immunogen for this XBP1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the XBP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the XBP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1

Background: XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response