YAP1 antibody (AA 420-446)

Catalog No. ABIN3029661

This anti-YAP1 antibody is a Rabbit Polyclonal antibody detecting YAP1 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for YAP1 antibody (AA 420-446) (ABIN3029661)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-YAP1 Antibody

Binding Specificity: AA 420-446

Purification: Purified

Immunogen: A portion of amino acids 420-446 from human YAP1 was used as the immunogen for this YAP antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the YAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP

Background: YAP is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. [UniProt]

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha