NEFH antibody

Catalog No. ABIN4956221

This Chicken Polyclonal antibody specifically detects NEFH in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Rat, Pig and Cowand has been mentioned in 1 publication.

Quick Overview for NEFH antibody (ABIN4956221)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Pig, Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NEFH Antibody

Specificity: On western blots CPCA-NF-H recognizes NF-H from all mammalian species tested to date very strongly and shows some weaker reactivity with NF-M. The most immunogenic regions of NF-H and NF-M are the phosphorylated KSP type repeats, and this antibody shows very strong reactivity with these.

Characteristics: To raise this particular antibody bovine intermediate filaments were prepared fromspinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis on a Biorad Prepcell. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. The resulting polyclonal antibody belongs to the IgY subclass. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.

Isotype: IgY

Application Details

Application Notes: The IgY solution has an extremely high titer and can be used at dilutions of at least 1:100,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000,000 or lower. We supply aliquots of 50 µL, which should be enough for thousands of experiments.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeat freezing and thawing

Storage: 4 °C/-20 °C

References for anti-NEFH antibody (ABIN4956221)

Picchiarelli, Demestre, Zuko, Been, Higelin, Dieterlé, Goy, Mallik, Sellier, Scekic-Zahirovic, Zhang, Rosenbohm, Sijlmans, Aly, Mersmann, Sanjuan-Ruiz, Hübers, Messaddeq, Wagner, van Bakel et al.: "FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis. ..." in: Nature neuroscience, Vol. 22, Issue 11, pp. 1793-1805, (2020) (PubMed).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Background: Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. The HGNC name for this protein is NEFH.