Complement C3b Alpha' Chain antibody

Catalog No. ABIN4956351

Product Details

Target: Complement C3b Alpha' Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Cross-Reactivity: Human

Purification: purified

Immunogen: human complement component C3

Clone: H206

Isotype: IgG1

Application Details

Application Notes: Dilution for IHC: 1:10

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL deionized water

Buffer: PBS, pH 7.22

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Target Details

Target: Complement C3b Alpha' Chain

Alternative Name: C3b-alpha

Background: H 206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c). Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-ß, (H11, Cat. No. 61020) and negative control anti-C3a (H13, Cat. No. 61018). Activated, deposited C3b bears C3b-α and C3b-ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with H 13.