SYNPO antibody (N-Term)

Catalog No. ABIN4956542

The Guinea Pig Polyclonal anti-SYNPO antibody has been validated for WB, IHC and IF. It is suitable to detect SYNPO in samples from Mouse.

Quick Overview for SYNPO antibody (N-Term) (ABIN4956542)

Target: SYNPO (Synaptopodin (SYNPO))

Reactivity: Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SYNPO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SYNPO Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse (Murine)

Immunogen: Synthetic peptides (mouse internal N-terminal sequence)

Application Details

Application Notes: Dilution for IHC: 1:50, for WB 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: serum

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Target Details for SYNPO

Target: SYNPO (Synaptopodin (SYNPO))

Alternative Name: synaptopodin

Background: The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding proteins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to post-translational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin. 1. The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. 2. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-depen dent expression in cultured hippocampal neurons.