AGO1 antibody (N-Term) (HRP)

Catalog No. ABIN4966093

This Rabbit Polyclonal antibody specifically detects AGO1 in WB. It exhibits reactivity toward Arabidopsis thaliana and Nicotiana benthamiana.

Quick Overview for AGO1 antibody (N-Term) (HRP) (ABIN4966093)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Arabidopsis thaliana, Nicotiana benthamiana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO1 antibody is conjugated to HRP

Application: Western Blotting (WB)

Product Details anti-AGO1 Antibody (HRP)

Binding Specificity: N-Term

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii

Predicted Reactivity: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera

Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.

Application Details

Application Notes: 1: 1000 with enhanced ECL (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage Comment: Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: argonaute 1 (AGO1)

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Horseradish Peroxidase (HRP).

Molecular Weight: 116.4 | 130 kDa

UniProt: O04379

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization