Cytochrome C antibody

Catalog No. ABIN4966146

This anti-Cytochrome C antibody is a Rabbit Polyclonal antibody detecting Cytochrome C in WB. Suitable for Arabidopsis thaliana.

Quick Overview for Cytochrome C antibody (ABIN4966146)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cytochrome C Antibody

Cross-Reactivity (Details): Not reactive in: Arabidopsis thaliana CytC6

Predicted Reactivity: cytc1 and cytc2 from following species: A. theoprasi, B. napus, B. oleracea, C. maxima, Ch. reinhardtii (peptide target partially conserved), L. esculentum, Lupinus luteus, M. truncatula, O. sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrella patens, R. comunis, S. nigra, V. vinifera

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2) , TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)

Application Details

Application Notes: 1: 5000 with standard ECL (WB)

Comment:

The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Storage Comment: store lyophilized at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles and store at -80°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: cytochrome c (Cyt c)

Background: Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.

Molecular Weight: 12,5 | 14 kDa for A. thaliana

UniProt: D7KMK0, D7LY03

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity