NRT2:1 antibody (C-Term)

Catalog No. ABIN4966259

The Rabbit Polyclonal anti-NRT2:1 antibody is suitable to detect NRT2:1 in samples from Arabidopsis thaliana. It has been validated for WB.

Quick Overview for NRT2:1 antibody (C-Term) (ABIN4966259)

Target: NRT2:1 (Nitrate Transporter 2:1 (NRT2:1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-NRT2:1 Antibody

Binding Specificity: C-Term

Purpose: Anti-NRT2,1 | Nitrate transporter 2,1

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Alexandrium pacificum, Glycine max, Solanum tuberosum

Predicted Reactivity: Brassica juncea,Brassica napus,Brassuca rapa,Ricinus communis

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NRT2.1 C-terminal sequence

Application Details

Application Notes: 1 : 5000 (WB)

Comment:

Microsomal fraction is recommended to be used as it will have higher amount of NRT2.1 protein. If a total protein extract is used SDS should be applied from the beginning in extraction buffer as well as protease inhibitor coctail.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for NRT2:1

Target: NRT2:1 (Nitrate Transporter 2:1 (NRT2:1))

Alternative Name: Nitrate transporter 2.1 (NRT2.1)

Background: NRT2.1 is a high affinity nitrate transporter which is up-regulated by nitrate. Functions as a repressor of lateral root initiation independently of nitrate uptake. Synonymes: Protein ACH1, Protein LATERAL ROOT INITIATION 1, ACH1, ATNRT2.1, ATNRT2:1, LIN1, NITRATE TRANSPORTER 2, NITRATE TRANSPORTER 2.1, NITRATE TRANSPORTER 2:1, NRT2, NRT2.1, NRT2:1, NRT2,1AT

Molecular Weight: 57.7 | 45 kDa

Gene ID: 837327

UniProt: O82811