Phosphoglucomutase 1 antibody

Catalog No. ABIN4966274

The Rabbit Polyclonal anti-Phosphoglucomutase 1 antibody has been validated for WB. It is suitable to detect Phosphoglucomutase 1 in samples from Zea mays.

Quick Overview for Phosphoglucomutase 1 antibody (ABIN4966274)

Target: Phosphoglucomutase 1 (PGM1)

Reactivity: Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoglucomutase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phosphoglucomutase 1 Antibody

Predicted Reactivity: Arabidopsis thaliana, Glycine max, Triticum aestivum

Immunogen: Recombinant phosphoglucomutase of Zea mays, UniProt: P93804

Application Details

Application Notes: 1:1000 (WB)

Comment:

Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 μl suspension was washed with 200 μl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 μl primary antibodies in 180 μl TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 μl TTBS and hence a real incubation with 200 μl plant extract (supernatant 20,000 x g for 3 min.), 200μl of TTBS and further 50 μl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 μl TTBS were performed prior mixing with 100 μl SDS-Sample buffer.Antibody is recognizing recombinant PGM1, overexpressed in E.coli.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Phosphoglucomutase 1

Target: Phosphoglucomutase 1 (PGM1)

Alternative Name: Phosphoglucomutase, cytoplasmic 1 (PGM1)

Background: Phosphoglucomutase is an enzyme which participates in both the breakdown and synthesis of glucose.

Molecular Weight: 63 kDa

UniProt: P93804

Pathways: Cellular Glucan Metabolic Process