HY5 antibody
The Rabbit Polyclonal anti-HY5 antibody has been validated for WB. It is suitable to detect HY5 in samples from Arabidopsis thaliana.
Catalog No. ABIN4966286
Quick Overview for HY5 antibody (ABIN4966286)
Target
HY5
(Transcription Factor HY5 (HY5))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: Oryza sativa, Pisum sativum, Populus sp.
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Predicted Reactivity
- Brassica pekinensis
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Immunogen
- KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646 , TAIR: AT5G11260. Chosen peptide is not conserved in HYH protein sequence.
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Application Notes
- 1: 1000 (WB)
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Comment
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This antibody detects well recombinant HY5 in Nicotiana benthamiana (image below) while a signal in endogenous total cell extract of Arabidopsis thaliana has not been optimised in applied conditions (as below).Using nuclear fraction for detection of endogenous protein is recommened, however it has not been optimized yet.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please add 50 μL of sterile water For reconstitution
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Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- HY5 (Transcription Factor HY5 (HY5))
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Alternative Name
- Protein long hypocotyl 5 (HY5)
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Background
- HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.
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Molecular Weight
- 18.5 kDa
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UniProt
- O24646
Target
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