RBOHD antibody

Catalog No. ABIN4966293

The Rabbit Polyclonal anti-RBOHD antibody (ABIN4966293) specifically detects RBOHD in IF and WB. The antibody is reactive with Arabidopsis thaliana and Tomato samples.

Quick Overview for RBOHD antibody (ABIN4966293)

Target: RBOHD (Respiratory Burst Oxidase-D (RBOHD))

Reactivity: Arabidopsis thaliana, Tomato

Host: Rabbit

Clonality: Polyclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Product Details anti-RBOHD Antibody

Purpose: Anti-RBOHD | Respiratory burst oxidase homolog protein D

Cross-Reactivity (Details): Arabidopsis thaliana, Solanum sp.
Not reactive in: Marchantia polymorpha, Medicago sativa

Predicted Reactivity: Brassica napus,Citrus sinensis,Citrullus colocynthis,Cucumis melo,Fragaria ananassa,Gossypium arboreum,Hordeum vulgare,Manihot esculenta,Morus notabilis,Nicotiana tabacum,Oryza sativa,Picea abies,Ricinus communis,Sinensis osbeck,Solanum tuberosum,Theobroma cacao,Triticum aestivum,Zea mays,Zostera marina

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide chosen from Arabidopsis thaliana RBOHD sequence

Application Details

Application Notes: 1 : 1000 (WB)

Comment:

RBOHD is a protein with 6 transmembrane-spanning regions, heat denaturation will cause protein aggregation. Therefore 30 minutes at RT was applied in the experiment below.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for RBOHD

Target: RBOHD (Respiratory Burst Oxidase-D (RBOHD))

Alternative Name: Respiratory burst oxidase homolog protein D (RBOHD)

Background: RBOHD (Respiratory burst oxidase homolog protein D) is a Calcium-dependent NADPH oxidase that generates superoxide. Is involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling.

Molecular Weight: 104 kDa

Gene ID: 834842

UniProt: Q9FIJ0