RNA Polymerase beta Subunit (Chloroplast) (RpoB) (Subunit beta) antibody

Catalog No. ABIN4966309

This Rabbit Polyclonal antibody specifically detects RNA Polymerase beta Subunit (Chloroplast) (RpoB) in WB. It exhibits reactivity toward Zea mays.

Quick Overview for RNA Polymerase beta Subunit (Chloroplast) (RpoB) (Subunit beta) antibody (ABIN4966309)

Target: RNA Polymerase beta Subunit (Chloroplast) (RpoB)

Reactivity: Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: Subunit beta

Purpose: Anti-RpoB | RNA polymerase beta subunit (chloroplast) (maize)

Cross-Reactivity (Details): Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Alloteropsis semialata,Campanula americana,Coleataenia prionitis,Digitaria exilis,Echinochloa crus-galli var. crus-galli,Eragrostis tef,Microlaena stipoides,Hordeum vulgare,Miscanthus sacchariflorus,Oryza sativa,Phragmites australis,Potamophila parviflora,Rhynchoryza subulata,Saccharum officinarum,Setaria italica,Sorghum bicolor,Stipa lipskyi,Sporobolus michauxianus,Triticum aestivum

Purification: Immunogen affinity purified serum

Immunogen: His-tagged

Application Details

Application Notes: 1 : 500 (WB)

Comment:

Antibody does not work on total cell extracts. Stromal fraction has to be used.This antibody is detecting recombinant RpoB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: RNA Polymerase beta Subunit (Chloroplast) (RpoB)

Background: RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.

Molecular Weight: 121 kDa