Rubisco Large Subunit, Form II (RbcL II) antibody

Catalog No. ABIN4966313

The Rabbit Polyclonal anti-Rubisco Large Subunit, Form II (RbcL II) antibody is suitable to detect Rubisco Large Subunit, Form II (RbcL II) in samples from Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus and Rhodospirillales. It has been validated for IF and WB.

Quick Overview for Rubisco Large Subunit, Form II (RbcL II) antibody (ABIN4966313)

Target: Rubisco Large Subunit, Form II (RbcL II)

Reactivity: Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillales

Host: Rabbit

Clonality: Polyclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Product Details

Purpose: Anti-RbcL II | Rubisco large subunit, form II

Cross-Reactivity (Details): Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillum rubrum
Not reactive in: Burkholderi, Cyanobium sp.

Predicted Reactivity: Acidithiobacillus ferrooxidans,Dechloromonas aromatica,Gonyaulax polyedra,Halothiobacillus neapolitanus,Leptothrix cholodnii,Magnetovibrio blakemorei,Rhodobacter sphaeroides,Thiobacillus denitrificans,Rhodopseudomonas palustrisAnd with a mismatch in one single amino acid: Gallionella capsiferriformans,Mariprofundus ferrooxydans,Thioalkalicoccus limnaeus,Methanomethylovorans hollandica,Methanococcoides burtonii,Methanosaeta concili,Methanolobus tindarius,Methanohalophilus mahii,and the dinoflagellate Symbiodinium sp. (ex Stylophora pistillata)

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide conserved in known RbcL form II protein sequences

Application Details

Application Notes: 1:10 000 (IF), (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Rubisco Large Subunit, Form II (RbcL II)

Background:

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. Form II Rubisco is present in many photosynthetic bacteria and archaea and in some photosynthetic dinoflagellates. The large subunit (LSU) of form I Rubisco is encoded by the RbcL/cbbL gene, while form II LSU is encoded by cbbM.

Additional information: The antibody does not detect Form I RbcL/cbbL

Molecular Weight: 51.7 kDa