Fertilization-Independent Endosperm antibody

Catalog No. ABIN4966398

The Rabbit Polyclonal anti-Fertilization-Independent Endosperm antibody (ABIN4966398) specifically detects Fertilization-Independent Endosperm in WB. The antibody is reactive with Arabidopsis thaliana samples.

Quick Overview for Fertilization-Independent Endosperm antibody (ABIN4966398)

Target: Fertilization-Independent Endosperm (FIE) (Polycomb Group Protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-Fertilization-Independent Endosperm Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arabidopsis thaliana, Triticum aestivum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740

Application Details

Application Notes: 1 : 1000 with ECL (WB)

Comment:

This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Fertilization-Independent Endosperm

Target: Fertilization-Independent Endosperm (FIE) (Polycomb Group Protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE))

Alternative Name: fertilization-independent endosperm (FIE)

Background: FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.

Molecular Weight: 41 kDa

UniProt: Q9LT47