RANBP1 antibody (N-Term)

Catalog No. ABIN497936

The Rabbit Polyclonal anti-RANBP1 antibody is suitable to detect RANBP1 in samples from Human, Mouse, Rat and Cow. It has been validated for WB and IF.

Quick Overview for RANBP1 antibody (N-Term) (ABIN497936)

Target: RANBP1 (RAN Binding Protein 1 (RANBP1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-RANBP1 Antibody

Binding Specificity: AA 1-187, N-Term

Specificity: Recognizes RAN binding protein 1/RANBP1 (1-187).

Cross-Reactivity (Details): Species reactivity (expected):Rat (97 %), Bovine (88 %).
Species reactivity (tested):Human, Mouse.

Purification: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 187 of Ran BP-1

Application Details

Application Notes: Suitable for use in Western blotting, and Immunocytochemistry/Immunofluorescence. Recommended Dilutions: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 10 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for RANBP1

Target: RANBP1 (RAN Binding Protein 1 (RANBP1))

Alternative Name: RANBP1

Background: Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92 % similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.Synonyms: Ran-binding protein 1, Ran-specific GTPase-activating protein

Gene ID: 5902, 9606

UniProt: P43487