LAMP2 antibody (C-Term)

Catalog No. ABIN497979

This anti-LAMP2 antibody is a Rabbit Polyclonal antibody detecting LAMP2 in WB, IF and EIA. Suitable for Human and Mouse.

Quick Overview for LAMP2 antibody (C-Term) (ABIN497979)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-LAMP2 Antibody

Binding Specificity: C-Term

Specificity: Recognizes CD107b/LAMP2 (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Immunoaffinity Chromatography

Immunogen: LAMP2 antibody was raised against a 17 amino acid peptide from near the carboxy of Human LAMP-2 (NP_054701).

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 1-2 μg/mL. Despite its predicted size, LAMP-2 migrates at 115 kDa in SDS-PAGE. Positive Control: HepG2 Cell Lysate. Immunocytochemistry. Positive Control: HepG2 Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: CD107b / LAMP2

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2

Gene ID: 3920

NCBI Accession: NP_054701

UniProt: P13473

Pathways: Autophagy