ATG16L1 antibody (N-Term)

Catalog No. ABIN497988

This anti-ATG16L1 antibody is a Rabbit Polyclonal antibody detecting ATG16L1 in WB, IF and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for ATG16L1 antibody (N-Term) (ABIN497988)

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG16L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ATG16L1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes ATG16 (N-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Immunoaffinity Chromatography

Immunogen: ATG16 antibody was raised against a 18 amino acid peptide from near the amino terminus of Human ATG16 (Genbank accession No. NP_110430).

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: ATG16 antibody can be used for the detection of ATG16 at 0.5-1 μg/mL. Immunocytochemistry. Positive Control: HeLa Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Alternative Name: APG16L / ATG16L1

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.Synonyms: APG16-like 1, Autophagy-related protein 16-1

Gene ID: 55054

NCBI Accession: NP_110430

UniProt: Q676U5

Pathways: Autophagy