POU2F3 antibody (Internal Region)

Catalog No. ABIN498032

The Goat Polyclonal anti-POU2F3 antibody is suitable to detect POU2F3 in samples from Human, Rat, Dog and Cow. It has been validated for WB and EIA.

Quick Overview for POU2F3 antibody (Internal Region) (ABIN498032)

Target: POU2F3 (POU Class 2 Homeobox 3 (POU2F3))

Reactivity: Human, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This POU2F3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-POU2F3 Antibody

Binding Specificity: Internal Region

Sequence: C-EPHLEASQHL PVPKH

Specificity: Recognizes POU2F3.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_055167.2. Genename: POU2F3

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEK293 andHepG2 (calculated MW of 47.0 kDa according to NP_055167.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POU2F3

Target: POU2F3 (POU Class 2 Homeobox 3 (POU2F3))

Alternative Name: POU2F3

Background: POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes.Synonyms: OTF-11, OTF11, Oct-11, Octamer-binding protein 11, Octamer-binding transcription factor 11, PLA-1, PLA1, POU domain class 2 transcription factor 3, Skn-1

Gene ID: 25833

NCBI Accession: NP_055167

UniProt: Q9UKI9