POU3F2 antibody (Internal Region)

Catalog No. ABIN498033

The Goat Polyclonal anti-POU3F2 antibody (ABIN498033) specifically detects POU3F2 in WB and EIA. The antibody is reactive with Human, Rat, Mouse, Cow and Pig samples.

Quick Overview for POU3F2 antibody (Internal Region) (ABIN498033)

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This POU3F2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-POU3F2 Antibody

Binding Specificity: Internal Region

Sequence: C-AQSLVQGDYG ALQSN

Specificity: Recognizes POU3F2/BRN2/OCT7.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine and Porcine.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (near N Terminus) of the protein sequence according to NP_005595.2 Genename: POU3F2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-3 μg/mL. Approx 45 kDa band observed in Human Brain (Cerebellum) andin Mouse and Rat Brain lysates (calculated MW of 46.5 kDa according to HumanNP_005595.2 and 47.5 kDa according to Mouse NP_032925.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Alternative Name: POU3F2

Background: POU3F2 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.Synonyms: BRN2, Brain-2, Brain-specific homeobox/POU domain protein 2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, OCT7, OTF-7, OTF7, Oct-7, Octamer-binding protein 7, Octamer-binding transcription factor 7, POU domain class 3 transcription factor 2

Gene ID: 5454

NCBI Accession: NP_005595

UniProt: P20265